Acetonitrile : Water 50:50 Ratio HPLC is a ready-to-use binary solvent mixture formulated for liquid chromatography. It supports HPLC and LC-MS methods requiring a consistent solvent composition.
Acetonitrile Water 50:50 Ratio HPLC | CAS: 75-05-8 | M.F: CH3CN | MW: 41.05. , Buy now for reliable and accurate results from LiChrom HPLC Solvents.
Acetonitrile HPLC is a highly polar organic solvent that is clear, colorless, and has an ether-like odor. When mixed with water, the acetonitrile concentration becomes diluted. In this process, it leads to improved chromatographic performance, especially of early-eluting, hydrophilic peptides. Additionally, it is ultra-filtrated to reduce particulate contamination.
Applications
- Acetonitrile: Water 50:50 ratio HPLC acts as the mobile phase in both LC-MS and HPLC methods.
- This solution can also be used as the washing solution for needles or injection ports.
- It is most commonly used as the mobile phase in reverse-phase chromatography.
Key Features
- 50:50 Acetonitrile Water ratio optimized for high solubility and performance
- Low UV absorbance for transparent baselines in detection systems
- Low viscosity - Enables superior system pressure performance and extends pump lifeExcellent for reproducible, reliable results
General Overview
The Acetonitrile: Water 50:50 Ratio HPLC is a ready-to-use, high-purity binary solvent mixture that is suitable for use in high-performance liquid chromatography (HPLC). Specifically composed of 50% Acetonitrile and 50% Water, this HPLC-grade composition offers high-resolution, accurate, and reproducible analytical results on a routine basis.
Need to know where to buy Acetonitrile: Water 50:50 Ratio HPLC or its price? You can get it from LiChrom, your trusted laboratory reagents and solvents supplier. Certificate of Analysis (COA), SDS (Safety Data Sheet), and bulk ordering price for research laboratories and industries are available on request. The prices vary based on quantity or the size of the package. We offer bulk discount orders and precise quotes. Get reliable solutions at a reasonable price without the quality or performance.
To obtain true findings in analytical separations, it requires a good formulation of Acetonitrile: Water 50:50 Ratio HPLC is required. Purchase the product now and achieve unmatched performance in solvents. In the case of LC-MS, up to the UV detection application, seek the quality experts.
About Brand
LiChrom products are designed specifically for Liquid Chromatography. LiChrom products exceed the rigorous requirement of HPLC analysis in various industries. Apart from HPLC- solvents, the LiChrom series encompasses HPLC-grade buffer salts, and HPLC ion pair reagents. These products provide the highest levels of accuracy and precision, as well as reproducibility of results,ensuring that your chromatography requirements will be met.
Frequently Asked Questions
The pre-mixed 50:50 composition provides a consistent solvent ratio without separate preparation of the components. This simplifies routine HPLC workflows and supports reproducible chromatographic conditions.
Acetonitrile : Water 50:50 Ratio HPLC can function as a mobile phase in reverse-phase chromatography. Its balanced organic and aqueous composition supports separation of suitable analytes.
Yes. This product is listed for use as a mobile phase in LC-MS methods. Its high-purity formulation and controlled composition support consistent analytical measurements.
An equal proportion of Acetonitrile and Water provides a ready-to-use mobile-phase composition for methods requiring this solvent strength. It also eliminates the need for separate preparation of the two components.
Yes. The formulation can function as a needle or injection-port wash solution, supporting routine HPLC instrument workflows in addition to mobile-phase applications.
The formulation provides: • 50:50 Acetonitrile-to-Water composition • High-purity formulation • Low UV absorbance • Low viscosity • Low non-volatile matter • Low fluorescence background • HPLC suitability
The product is supplied as a liquid and has an assay of ≥99.9%. The listed analytical characteristics include a refractive index of approximately 1.344, a boiling point of approximately 81–82°C, and defined UV absorption limits.
Acetonitrile : Water 50:50 Ratio HPLC is available in package options designed for routine HPLC, LC-MS, and higher-volume laboratory requirements. Current package availability can be checked through LiChrom.
Customers seeking this pre-mixed solvent for HPLC, LC-MS, reverse-phase chromatography, or analytical laboratory workflows can purchase it from LiChrom in the USA.
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